This study shows the effect of low-power laser irradiation on the proliferation activity of cells irradiated by a 830 nm semiconductor BTL-10 laser in a continuous or pulsed mode at an energy density ranging from 2 to 99 J/cm2 (power output, 72 to 360 mW). The irradiated cells were incubated and their proliferation activity was assessed by the MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) assay at 24, 48, 72 and 96 h. In comparison with the control populations, the irradiated cells showed a significant increase in proliferation, regardless of the energy density used, at 72 and 96 h but not at 24 and 48 h. In addition, the stimulation of proliferation was related to the mode of irradiation. The cells irradiated in the pulsed mode (5 000 Hz) showed a higher proliferation activity than the cells treated by continuous laser light. It is concluded that low-power lasers stimulate HeLa cell proliferation.
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